Help

The database of interacting GPCRs can be accessed by browsing the whole list or by searching for specific GPCRS using the two links in the navigation menu: 'Browse' and 'Search'.

  • Browse page provides a list of the interactions. The list consists of entries that contain a brief information about the interactions. The column definitions are listed below:

    • Type: Type of the interaction. Can be either Homodimer or Heterodimer. Clicking on this field opens the 'Interaction Details' page of that specific interaction.
    • Interacting GPCRs: The GPCR short names that are involved in the interaction. If the interaction type is Homodimer, this field contains only one GPCR name since there is only one type of GPCR in the interaction. If the interaction type is Heterodimer, this field contains two or more GPCR names. The GPCR names are clickable and provide a link for the 'GPCR Details' page for that specific GPCR.
    • Species: Name of the species that the interacting GPCRs belong to.
    • Interface: If the interaction contains any interface information, this field is written as 'Details' and can be clicked to list the interaction details as in the first column 'Type'. If this field is empty, that means the interaction do not contain interface information.
    • Experiment: The type of the experiment studied for this interaction. In can be 'Biological', 'Computational' or both. The experiment details are listed in the 'Interaction Details' page.
    • Pubmed ID: The related Pubmed Id of the published article that provides the interaction. Clicking on this field will direct to related article in Pubmed.
    • Year: Publication year of the article.

  • 'Interaction Details' and 'GPCR Details' pages are the other informative pages that can be reachable using the links in the listed table. Interaction Details page lists the following extra information in addition to the listed above information about the clicked interactions:

    • Interface: The interface details are listed in this field if they are included in the article. The interface can be interacting regions (Transmembrane, loops or C/N terminals) or specific interacting residues.
    • Interaction model: The model in the Jmol window. This interaction model is produced using a protein-protein docking server: ClusPro 2.0 (http://cluspro.bu.edu). The most meaningful model is selected.
    • Downloadable models: The other interaction models created by the ClusPro 2.0 server. These models are easily downloadable in .zip archive format file for further analysis.

    GPCR Details page lists the following information about the GPCR of interest:

    • ID: GPCRDB (http://www.gpcr.org/7tm) database ID of the GPCR. There is a link on this field to access the GPCRDB page.
    • Name: Short name of the GPCR.
    • Species: The species that this GPCR belongs to.
    • Access code: Uniprot (http://www.uniprot.org) access code of the GPCR. There is a link on this field to access the Uniprot page.
    • Family: Family hierarchy of the GPCR. Every ancestor family member has a link to easily access to their GPCRDB page.
    • Involved interactions: The table at the bottom of the page lists the interactions that this GPCR is involved in. The format of this table is the same as the table in the Browse page.

  • Search page is used to search the IntGPCR database for specific data. Results of the search are listed in the Browse page. The search can be performed by specifying the following parameters:

    • Interaction type: Specifies which type of interactions can be listed. The options are: All, Homodimers or Heterodimers.
    • GPCR: The names of the GPCRs are listed. A specific GPCR can be searched in the interacting GPCRs.
    • Species: The species of the GPCRs are listed.
    • Pubmed ID: A Pubmed ID can be entered in the specified textbox. If the related article is in the database, the interaction data contained in that article will be listed.
    • Experiment type: The type of the experiment can be specified as All, Biological or Computational.
    • Interface: The interface search parameter states if the interactions have interface data or not.

If you have specific questions or concerns, please mail to intgpcr@gmail.com.